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Microbiology For Himedia
Our product range includes a wide range of tryptone soya agar w/ added nacl, tergitol-7 agar base, r2a agar (agar medium s), chromogenic coliform agar, mannitol salt agar and hi crome listeria ottaviani agosti agar base.
Tryptone Soya Agar w/ added NaCl
Product Price: Rs 5,500 / PackGet Best Price
Minimum Order Quantity: 1 Pack
Product Brochure
Product Details:
| Packaging Size | 100 g |
| Product Code | M1969 |
| Brand | himedia |
| CAS Number | 7647-14-5 |
| Shelf Life | 4 years |
| Country of Origin | Made in India |
- Purpose: A highly nutritious general-purpose medium recommended for use when 1% Sodium Chloride is needed in the medium.
- Composition:
- Tryptone: 15.0 g/L
- Soya peptone: 5.0 g/L
- Sodium chloride: 10.0 g/L
- Agar: 15.0 g/L
- Principle:
- Tryptone and Soya peptone provide nitrogenous compounds and other growth factors.
- The medium contains a slightly high concentration of sodium chloride, which provides essential ions and maintains osmotic equilibrium.
- Instructions for Use:
- Suspend 45.0 grams in 1000 ml purified/distilled water.
- Heat to boiling to dissolve completely.
- Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
- Quality Control Specifications:
- Appearance of powder: Yellow-colored homogeneous free-flowing powder.
- Appearance of prepared medium: Yellow-colored clear gel forms in Petri plates.
- pH (at 25°C): 7.3±0.2
- Storage: Keep the dehydrated powder in tightly-sealed containers between 25-30°C, protected from direct sunlight. Shelf life is 4 years under optimal conditions.
- Disposal: Sterilize prepared plates and other contaminated materials before discarding
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Tergitol-7 Agar Base
Product Price: Rs 5,400 / PackGet Best Price
Product Brochure
Product Details:
| Packaging Size | 500 g |
| Manufacturer | HIMIEDA |
| Prescription/Non prescription | Prescription |
| Final pH | 6.50 - 7.50 |
| Storage Temperature | below 30 DegreeC |
| Product Code | M616 |
| Melting Temp | 38-40C |
| Brand | HIMIEDA |
| CAS Number | 9002-18-0 |
| Shelf Life | 36 Months. |
| Country of Origin | Made in India |
1. Purpose: Tergitol-7 Agar Base is used for selective enumeration and identification of coliform organisms.
2. Selective Medium: It helps detect coliform bacteria and allows for early detection of E. coli in water analysis.
3. Composition: The base contains Sodium heptadecyl sulphate (Tergitol-7), which inhibits gram-positive bacteria.
4. Modification: Chapman modified the original formula by adding Triphenyl Tetrazolium Chloride (TTC), making the medium selective and differential1.
5. Recommended Use: Tergitol-7 Agar Base is recommended by the ISO Committee for coliform detection and enumeration
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R2A Agar (Agar Medium S)
Product Price: Rs 12,000 / PieceGet Best Price
Product Brochure
Product Details:
| Physical State | Powder |
| Purity | 99% |
| Packaging Size | 500GM |
| Packaging Type | Bottle |
| Category | Simple Media |
| Shelf Life | 36 Months. |
| Country of Origin | Made in India |
- Purpose: Developed for studying bacteria that inhabit potable water.
- Nutrient Content: Low, which stimulates slow-growing and chlorine-tolerant bacteria.
- Application:
- Used for qualitative and quantitative heterotrophic plate counts in treated potable water samples.
- Superior to Plate Count Agar for pour plate, spread plate, or membrane filter techniques.
- Composition:
- Peptone: Essential amino acids and nitrogen
- Dextrose: Carbon source
- Yeast extract: B-complex vitamins and growth enhancer
- Soluble starch: Neutralizer
- Sodium pyruvate: Stimulates stressed bacteria growth
- Magnesium sulfate and dipotassium phosphate: Buffering agents
- Recommended by: American Public Health Association (APHA) in Standard Methods for Water and Wastewater Examination
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Chromogenic Coliform Agar
Product Price: Rs 84,187 / KgGet Best Price
Minimum Order Quantity: 1 Kg
Product Brochure
Product Details:
| Packaging Size | 500 g |
| Manufacturer | Himedia |
| Composition | enzymatic digest of casein, yeast extract, sorbitol and sodium pyruvate |
| Treatment | drinking water, disinfected pool water or finished water from treatment plants. |
| Prescription/Non prescription | Prescription |
| Final pH | 6.8 |
| Storage Temperature | 15-25 DegreeC |
| Storage Temp | 2-8 DegreeC |
| Product Code | M1991i |
| Brand | HIMIEDA |
| CAS Number | 1473387-52-8 |
| Shelf Life | 24 Months |
| Country of Origin | Made in India |
- Purpose: CCA is a selective agar medium used to detect coliform bacteria in water and other samples.
- Differentiation: It contains chromogenic substrates that allow for distinguishing between coliform and non-coliform bacteria based on their ability to produce specific enzymes.
- Enzyme Indicators:
- Coliform Bacteria: Produce β-D-galactosidase, resulting in pink to red colonies.
- E. coli: Produce β-D-glucuronidase, leading to dark blue to violet colonies.
- Composition (per liter):
- Tryptone: 1 g
- Yeast extract: 2 g
- Sodium chloride: 5 g
- Sodium dihydrogen phosphate: 2.2 g
- Disodium hydrogen phosphate: 2.7 g
- Sodium pyruvate: 1 g
- Sorbitol: 1 g
- 6-chloro-3-indoxyl β-D-galactopyranoside: 0.2 g
- 5-bromo-4-chloro-3-indoxyl β-D-glucuronic acid: 0.1 g
- IPTG (Isopropyl-β-D-thiogalactopyranoside): 0.1 g
- Agar: 15 g
- Final pH: 6.8±0.2 at 25°C1.
- Application: Used for microbiological quality testing of water and food samples to detect coliforms
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Mannitol Salt Agar
Product Price: Rs 2,160 / PackGet Best Price
Product Brochure
Product Details:
| Packaging Size | 100 g |
| Manufacturer | HIMIEDA |
| Treatment | isolating pathogenic staphylococci from samples,cosmetics,and microbial limit tests. |
| Prescription/Non prescription | Prescription |
| Final pH | 7.4 |
| Product Code | MH118 |
| Brand | HIMIEDA |
| CAS Number | 143-74-8 |
| Shelf Life | 36 MONTHS |
| Country of Origin | Made in India |
· Purpose: MSA is a commonly used selective and differential growth medium in microbiology.
· Selective Action:
· Contains 7.5% sodium chloride, which selects for bacteria that can tolerate high salt concentrations.
· Staphylococci species can grow on MSA due to their salt tolerance.
· Inhibits the growth of most other gram-positive and gram-negative bacteria.
· Differential Aspect:
· Staphylococcus aureus (pathogenic staphylococci) can ferment mannitol, changing the pH of the medium to acidic.
· MSA contains phenol red as a pH indicator.
· Acidic pH turns the medium yellow due to mannitol fermentation by S. aureus.
· Coagulase-negative staphylococci (CONS) do not ferment mannitol, so the medium remains pink around their colonies.
· Composition:
· Enzymatic digest of casein, animal tissue, and beef extract (sources of nutrients).
· D-Mannitol as the sole carbohydrate source.
· Sodium chloride (selective agent).
· Phenol red (pH indicator).
· Agar (solidifying agent).
· Application: Used to isolate and differentiate Staphylococcus spp., particularly S. aureus
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Hi Crome Listeria Ottaviani Agosti Agar Base
Product Price: Rs 5,900 / PieceGet Best Price
Minimum Order Quantity: 1 Piece
Product Brochure
Product Details:
| Physical State | Semi-Solid |
| Purity | 99% |
| Packaging Size | Plastic Bottle |
| Packaging Type | Bottle |
| Category | Complex Media |
| Color | White |
| Shelf Life | 36 MONTH |
| Country of Origin | Made in India |
-
- Purpose:
- HiCrome™ Listeria Ottaviani Agosti Agar Base is used for the selective and differential isolation of Listeria monocytogenes from food and animal feeds.
- This medium follows the method adopted by the ISO Committee for Listeria detection
- Composition:
- Contains the chromogenic compound 5-bromo-4-chloro-3-indolyl-β-D-glucopyranoside.
- This substrate detects the presence of β-glucosidase, which is common in all Listeria species.
-
- Appearance:
- Listeria colonies appear as blue-green on the medium due to β-glucosidase activity
-
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Violet Red Bile Glucose Agar
Product Price: Rs 9,962 / PackGet Best Price
Product Brochure
Product Details:
| Packaging Size | 500 g |
| Manufacturer | HIMEDIA |
| Composition | peptone, yeast extract, sodium chloride, glucose, bile salts, crystal violet, neutral red, agar |
| Treatment | used for the enumeration of Enterobacteriaceae in foods |
| Prescription/Non prescription | Prescription |
| Final pH | 7.4 |
| Product Code | MH581 |
| Brand | HIMEDIA |
| CAS Number | 302-95-4 |
| Shelf Life | 36 Months |
| Country of Origin | Made in India |
1. Purpose: VRBGA is a selective medium used for the detection and enumeration of bile-tolerant Gram-negative bacteria in various samples, including food, water, and other materials of sanitary importance
2. Composition:
o Peptic digest of animal tissue: Provides carbon, nitrogen, vitamins, and other essential growth nutrients.
o Yeast extract: Additional source of nutrients.
o Sodium chloride: Maintains osmotic equilibrium.
o Bile salts mixture: Inhibits accompanying Gram-positive flora.
o Crystal violet: Further inhibits Gram-positive organisms, especially Staphylococci.
o Glucose: The fermentable carbohydrate.
o Neutral red: Acts as a pH indicator
3. Result Interpretation:
o Purple colonies surrounded by purple haloes: Rapid lactose fermenters (e.g., coliform bacteria like E. coli).
o Pale colonies with greenish zones: Non-fermenters or late lactose fermenters.
o Pink pin-point colonies: Possibly Enterococci
o Colorless colonies: Lactose-negative Bacteria
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Orange Serum Agar
Product Price: Rs 12,187 / PackGet Best Price
Product Brochure
Product Details:
| Packaging Size | 500 g |
| Manufacturer | HIMEDIA |
| Prescription/Non prescription | Prescription |
| Final pH | 5.4 - 5.6 |
| Product Code | M933 |
| Brand | HIMIEDA |
| CAS Number | 110673. |
| Shelf Life | 36 Months. |
| Country of Origin | Made in India |
-
- Purpose: Orange Serum Agar is specifically designed for the isolation and enumeration of microorganisms that can survive in citrus products.
- Microorganisms Targeted: It helps detect acid-tolerant bacteria, molds, and yeasts commonly associated with spoilage in citrus fruit products.
- Low pH Environment: The low pH of citrus juices limits the growth of microorganisms to those capable of tolerating the acidic conditions.
- Composition:
- Tryptone: Provides amino acids and complex nitrogenous substances.
- Glucose: Serves as a fermentable carbohydrate and energy source.
- Yeast extract: Supplies vitamins to stimulate growth.
- Orange serum: Creates an optimal environment for acid-tolerant microorganisms.
- Superiority: In comparative studies, Orange Serum Agar was found to be superior to other media for total count and detection of spoilage organisms1.
- Application: Used in laboratories to cultivate and enumerate acidic microorganisms associated with citrus product spoilage
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Tryptone Soya Agar (Casein Soyabean Digest Agar) (Soyabean Casein Digest Agar)
Product Price: Rs 2,375 / KgGet Best Price
Product Brochure
Product Details:
| Packaging Size | 100 g |
| Manufacturer | HIMIEDA |
| Composition | Pancreatic digest of casein 15.0; Papaic digest of soya bean 5.0; Sodium chloride 5.0; Agar-Agar 15. |
| Treatment | determining efficiency of sanitization of containers, equipment surfaces, water miscible cosmetics |
| Final pH | 8.0 |
| Storage Temperature | 10-30 DegreeC |
| Product Code | M290 |
| Melting Temp | 45-50 DegreeC |
| Brand | HIMIEDA |
| CAS Number | 7647-14-5 |
| Shelf Life | 4 years |
| Country of Origin | Made in India |
- Purpose: TSA is a general-purpose agar medium that supports the growth of a wide variety of microorganisms.
- Composition:
- Contains two peptones derived from casein and soybean meal.
- Provides essential nutrients, including organic nitrogen (amino acids and peptides).
- Sodium chloride maintains osmotic balance.
- Agar serves as the solidifying agent.
- Uses:
- Microbial Cultivation: TSA is used for isolating and cultivating bacteria.
- Storage and Transportation: Suitable for maintaining stock cultures.
- Clinical Microbiology: Limited use due to not supporting fastidious bacteria.
- Hemolysis Testing: With added blood, it can test for hemolysis
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Macconkey Agar Himedia
Product Price: Rs 4,000 / GramGet Best Price
Product Details:
| Packaging Size | 500 g |
| Storage Temperature | 2-8degree celcius |
| Storage Temp | 2-8degree celcius |
| Product Code | M008 |
| Country of Origin | Made in India |
MacConkey Agar is a selective and differential medium developed for the isolation and differentiation of Gram-negative enteric bacilli, especially the Enterobacteriaceae family based on lactose fermentation. It is widely used in clinical, food, water, and pharmaceutical microbiology.
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Sabouraud Chloramphenicol Agar
Product Price: Rs 1,050 / PackGet Best Price
Product Details:
| Packaging Size | 100 g |
| Manufacturer | HIMIEDA |
| Product Code | M1067 |
| Brand | Himedia |
| Shelf Life | 36 Months. |
| Country of Origin | Made in India |
- Purpose: Used for the selective cultivation of yeasts and molds, especially from clinical and non-clinical samples.
- Composition:
- Peptones (casein and meat)
- Dextrose (glucose)
- Agar
- Chloramphenicol (antibiotic)
- Preparation:
- Suspend 65 grams in 1000 ml distilled water.
- Heat to boiling to dissolve completely.
- Sterilize by autoclaving at 121°C for 15 minutes.
- Uses:
- Isolates pathogenic fungi from samples with large numbers of other fungi or bacteria.
- Commonly used in medical, environmental, and food microbiology.
- Appearance: Clear to slightly opalescent, light amber gel.
- pH: 5.6 ± 0.2 at 25°C
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Blood Agar Base
Product Price: Rs 4,200 / KgGet Best Price
Product Details:
| Packaging Size | 500 g |
| Manufacturer | HIMIEDA |
| Prescription/Non prescription | Prescription |
| Product Code | M073 |
| Brand | HIMIEDA |
| CAS Number | 110886 |
| Shelf Life | 36 Months |
| Country of Origin | Made in India |
- Definition: Blood Agar Base is a nutrient-rich medium used to grow bacteria, especially those that are fastidious (require specific nutrients).
- Composition: Contains peptone, tryptose, sodium chloride, and agar. Typically enriched with 5% defibrinated mammalian blood (e.g., sheep, horse).
- Purpose:
- Supports the growth of a wide range of bacteria.
- Differentiates bacteria based on their hemolytic properties (ability to break down red blood cells).
- Types of Hemolysis:
- Alpha Hemolysis: Partial hemolysis, greenish discoloration.
- Beta Hemolysis: Complete hemolysis, clear zone around colonies.
- Gamma Hemolysis: No hemolysis, no change in the medium.
- Uses:
- Cultivating fastidious organisms like Streptococcus species.
- Identifying hemolytic activity of bacteria.
- Preparation:
- Dissolve the base in water, autoclave, cool, and add sterile blood.
- Pour into sterile plates for use.
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Chloramphenical Yeast Glucose Agar
Product Price: Rs 4,800 / KgGet Best Price
Product Details:
| Packaging Size | 500 g |
| Manufacturer | Himedia |
| Product Code | M1008 |
| Brand | Himedia |
| CAS Number | 56-75-7. |
| Shelf Life | 36 Months. |
| Country of Origin | Made in India |
- Purpose: Selective isolation and enumeration of yeasts and molds.
- Composition: Contains yeast extract, glucose, and chloramphenicol.
- Inhibition: Chloramphenicol inhibits bacterial growth.
- Application: Commonly used in dairy product analysis.
- Preparation: Dissolve 40.1 grams in 1 liter of distilled water and sterilize by autoclaving
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Fraser Broth Base
Product Price: Rs 6,550 / PieceGet Best Price
Product Details:
| Physical State | Powder |
| Purity | 99% |
| Packaging Size | 500ML |
| Packaging Type | Bottle |
| Shelf Life | 36 Months. |
| Country of Origin | Made in India |
| Brand | HIMIEDA |
- Purpose: Fraser Broth Base is a liquid medium used with supplements for the selective enrichment of L. monocytogenes and Listeria spp. from food, animal feeding, and environmental samples in the food production and handling area.
- Composition (per liter):
- Proteose peptone: 5.0 g
- Tryptone: 5.0 g
- Lab-Lemco powder: 5.0 g
- Yeast extract: 5.0 g
- Sodium chloride: 20.0 g
- Di-sodium hydrogen phosphate: 12.0 g
- Potassium dihydrogen phosphate: 1.35 g
- Aesculin: 1.0 g
- Lithium chloride: 3.0 g
- pH: 7.2 ± 0.2 at 25°C.
- Supplements:
- Fraser Supplement: Contains ferric ammonium citrate, nalidixic acid, and acriflavine hydrochloride.
- Half Fraser Supplement: Used for primary enrichment when combined with Fraser Broth
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Baird Parker Agar Base
Product Price: Rs 4,930 / PackGet Best Price
Minimum Order Quantity: 1 Pack
Product Details:
| Packaging Size | 500 g |
| Manufacturer | HImedia |
| Composition | Tryptone, HM peptone B and yeast extract are sources of nitrogen, carbon, sulphur, and vitamins. |
| Treatment | not intended for use in diagnosis, treatment, or prevention of disease in humans |
| Product Code | CM0961B |
| Brand | HImedia |
| Melting Temp | 100C |
| CAS Number | 147-14-8 |
| Shelf Life | 4 years |
| Country of Origin | Made in India |
· Chemical Name: Baird Parker Agar Base
· Use: Selective enumeration medium for Staphylococcus aureus.
· Composition:
· Tryptone: Provides nitrogen, carbon, sulfur, and vitamins.
· HM Peptone B: Source of nutrients.
· Yeast extract: Supplies carbon, nitrogen, and vitamins.
· Glycine: Enhances Staphylococcus growth.
· Sodium pyruvate: Stimulates S. aureus growth.
· Lithium chloride: Inhibits most contaminants.
· Agar: Solidifying agent.
· Principle: Correlates coagulase test with lipolysis zone; black colonies indicate tellurite reduction by coagulase-positive Staphylococci.
· Result Interpretation: Grey-black colonies and a clear zone are diagnostic for coagulase-positive Staphylococci.
· Preparation: Dissolve 63.0 grams in 950 ml distilled water, sterilize, and add egg yolk emulsion and potassium tellurite solution
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Tryptone Soya HiVeg Broth Sterile Powder
Product Price: Rs 6,850 / KgGet Best Price
Product Details:
| Packaging Size | 500 g |
| Manufacturer | HIMIEDA |
| Composition | Nutrient-rich: Supports the growth of a wide variety of microorganisms. |
| Prescription/Non prescription | Prescription |
| Product Code | MV011G-500G |
| Brand | HIMIEDA |
| CAS Number | 9002-18-0 |
| Shelf Life | 36 Months |
| Country of Origin | Made in India |
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Himedia Cetrimide Agar
Product Price: Rs 1,000 / PieceGet Best Price
Product Details:
| Physical State | Powder |
| Packaging Size | 100 g |
| Final pH | 0.7-0.2 |
| Storage Temperature | 10 - 30 degree celcius |
| Product Code | MH024-100G0.7-0.2 |
| Country of Origin | Made in India |
| Packaging Type | Plastic Jar |
Cetrimide Agar is a selective medium used for the isolation of Pseudomonas aeruginosa, particularly from pharmaceutical products. It contains cetrimide, which inhibits the growth of other bacteria and allows the selective growth of Pseudomonas species.
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Tcbs Agar Himedia
Product Price: Rs 5,400 / KgGet Best Price
Product Brochure
Product Details:
| Packaging Size | 500 g |
| Manufacturer | HIMIEDA |
| Product Code | M189 |
| Brand | HIMIEDA |
| Shelf Life | 36 Months. |
- Purpose: Selective isolation of Vibrio cholerae and other enteropathogenic Vibrios
- Composition: Contains peptone, yeast extract, sodium citrate, sodium thiosulfate, sodium cholate, oxgall, sucrose, sodium chloride, ferric citrate, bromothymol blue, thymol blue, and agar
- pH Level: Alkaline pH of 8.8 ± 0.2 to enhance recovery of Vibrio species
- Appearance: Light yellow to light tan homogeneous free-flowing powder
- Preparation: Dissolve in distilled water, heat to boiling, and cool to 50°C before use. Do not autoclave
- Usage: Ideal for laboratory use in microbiology for isolating and identifying Vibrio species
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Special Infusion Agar
Product Price: Rs 2,162.5 / KgGet Best Price
Minimum Order Quantity: 1 Kg
Product Brochure
Product Details:
| Packaging Size | 100 g |
| Product Code | M211 |
| Melting Temp | 90C |
| Brand | HIMEDIA |
| CAS Number | 7558-79-4 |
| Shelf Life | 4 years |
| Country of Origin | Made in India |
- Purpose:
- BHI Agar is a general-purpose medium used for the primary isolation of aerobic bacteria from clinical specimens.
- It is highly nutritious and can support luxuriant growth of a wide variety of microorganisms.
- Composition:
- BHI Agar derives its nutrients from brain heart infusion, peptone, and glucose components.
- Protease peptone and infusions serve as sources of carbon, nitrogen, vitamins, amino acids, and essential growth factors.
- Enrichment and Selectivity:
- BHI Agar can be further enriched by the addition of blood.
- It can also be rendered selective by adding different antibiotics.
- Applications:
- Cultivation of fastidious pathogenic bacteria, yeasts, and moulds.
- Widely used in clinical laboratories for bacterial isolation
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Himedia Mueller Hinton Agar
Product Price: Rs 4,500 / KgGet Best Price
Product Details:
| Packaging Size | 500 g |
| Manufacturer | HIMIEDA |
| Brand | HIMIEDA |
| Shelf Life | 36 Months. |
| Country of Origin | Made in India |
- Purpose: Used for antimicrobial susceptibility testing, particularly with the disk diffusion method
- Composition: Contains beef infusion, casein hydrolysate, starch, and agar
- pH Level: Maintained at 7.3 ± 0.1 at 25°C for optimal microbial growth
- Applications: Suitable for testing a wide range of non-fastidious bacterial pathogens
- Reproducibility: Known for consistent performance and reliable results
- Preparation: Easy to prepare by dissolving in distilled water and sterilizing by autoclaving
- Low Inhibitors: Designed to be low in sulfonamide, trimethoprim, and tetracycline inhibitors
- Standards: Recommended by CLSI (Clinical and Laboratory Standards Institute) for standardized testing
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Simmons Citrate Agar
Product Price: Rs 4,500 / KgGet Best Price
Product Brochure
Product Details:
| Packaging Details | Plastic Bottle |
| Purity | 99% |
| Packaging Size | 500 gm |
| Packaging Type | Bottle |
| Usage/Application | Laboratory |
| Brand | HIMIEDA |
| CAS Number | 7722-76-1. |
| Shelf Life | 60 Month |
| Country of Origin | Made in India |
- Purpose:
- Simmons Citrate Agar is a differential medium that helps distinguish bacteria based on their ability to utilize citrate as their sole source of carbon.
- It is commonly used in laboratories to gain insights into the metabolic capabilities of different bacterial species.
- Composition:
- Contains sodium citrate as the sole carbon source.
- Ammonium dihydrogen phosphate serves as the sole nitrogen source.
- Other components include sodium chloride, dipotassium phosphate, magnesium sulfate, bromothy mol blue (pH indicator), and agar (solidifying agent).
- Principle:
- Organisms capable of utilizing citrate will grow unrestricted on this medium.
- Bacteria that can metabolize citrate produce an enzyme called citrate-permease, which converts citrate to pyruvate.
- Pyruvate then enters the organism’s metabolic cycle for energy production.
- Growth on Simmons Citrate Agar indicates utilization of citrate.
- Uses:
- Differentiating Gram-negative bacteria based on citrate utilization.
- Identifying citrate-positive Salmonella species from citrate-negative ones.
- Aiding in the identification of Entero bacteriaceae.
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Pseudomonas Agar Base
Product Price: Rs 1,100 / PieceGet Best Price
Product Details:
| Purity | 99% |
| Category | Simple Media |
| Packaging Type | Bottle |
| Shelf Life | 36 Months. |
| Country of Origin | Made in India |
- Purpose: Used for the selective isolation of Pseudomonas species from various samples.
- Composition:
- Modification of King’s A Medium
- Contains magnesium chloride and potassium sulfate
- Selective Properties:
- Promotes pigment production
- Suppresses growth of Klebsiella, Proteus, and Providencia spp.
- Application Areas:
- Meat and dairy products
- Water
- Environmental samples
- Clinical specimens
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Buffered Sodium Chloride Peptone Solution
Product Price: Rs 2,090 / KgGet Best Price
Minimum Order Quantity: 1 Kg
Product Brochure
Product Details:
| Purity | 99% |
| Grade | Industrial |
| Usage/Application | as a diluent for carrying out microbial limit test from pharmaceutical products |
| Molecular Weight | 58.44 |
| Packaging Type | Plastic Bottle |
| Country of Origin | Made in India |
| Form | Powder |
- Purpose: Buffered Sodium Chloride Peptone Solution is primarily used for microbial limit testing in pharmaceutical products.
- Composition: The solution contains sodium chloride, peptone, and a phosphate buffer. The low peptone content helps maintain the viability of microorganisms without supporting their growth
- Applications:
- Microbial Examination: Used for the microbial examination of non-sterile pharmaceutical products
- Sample Preparation: Can be used as a solution for sample preparation, dilution, or washing
- Cosmetics: Conforms to ISO 21149 for the detection of viable microorganisms in cosmetics
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Mac conkey Agar
Product Price: Rs 4,000 / KgGet Best Price
Product Details:
| Grade | Chemical Grade |
| Form | Powder |
| Packaging Size | 500 gm |
| Usage/Application | for the isolation of gram-negative enteric bacteria and the differentiation of lactose fermenting fr |
| Brand | HIMIEDA |
| Color | Red |
| Shelf Life | 3 Years |
| Country of Origin | Made in India |
- Purpose: Selective and differential medium for isolating Gram-negative bacteria
- Applications: Commonly used to identify and differentiate Enterobacteriaceae family members
- Indicator: Neutral red (turns pink/red with lactose fermentation)
- Components: Peptone, lactose, bile salts, sodium chloride, neutral red, crystal violet, agar
- Preparation: Dissolve 49.53g in 1L distilled water, sterilize by autoclaving
- Storage: Store prepared plates at 2-8°C
- pH: Final pH of 7.1 ± 0.2 at 25°C
- Colony Appearance: Lactose fermenters appear pink/red; non-fermenters remain colorless
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MacConkey Broth Purple
Product Price: Rs 3,400 / KgGet Best Price
Product Details:
| Physical State | Powder |
| Usage/Application | Culture Media |
| Packaging Size | 500 g |
| Category | Simple Media |
| Grade Standard | Analytical Grade |
| Packaging Type | Plastic Bottle |
| Country of Origin | Made in India |
- Purpose: Differential medium for detecting coliforms
- Applications: Used in water and milk testing
- Indicator: Bromocresol purple (changes from purple to yellow with acid production)
- Components: Peptone, lactose, bile salts, sodium chloride
- Preparation: Dissolve 40g in 1L distilled water, sterilize by autoclaving
- Storage: Store prepared media below 8°C, dehydrated powder at 2-25°C
- pH: Final pH of 7.4 ± 0.2 at 25°C
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MGPY AGAR WITH COPPER
Product Price: Rs 6,000 / KgGet Best Price
Product Details:
| Grade | Industry Grade |
| Form | Powder |
| Packaging Size | 500 gm |
| Brand | HIMIEDA |
| Shelf Life | 3 Years |
| Country of Origin | Made in India |
- Composition:
- Agar: 20.0 g/L
- Cupric sulfate: 0.4 g/L
- Dextrose: 10.0 g/L
- Gelatin peptone: 5.0 g/L
- Malt extract: 3.0 g/L
- Yeast extract: 3.0 g/L
- pH Level: Final pH of 6.2 ± 0.2 at 25°C
- Form: Powder
- Application:
- Selective medium for isolating and cultivating wild yeasts in the brewing industry
- Preparation:
- Suspend 41.4 grams in 1000 ml distilled water.
- Heat to boiling to dissolve completely.
- Sterilize by autoclaving at 121°C for 15 minutes.
- Pour into sterile Petri plates.
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ECD Agar 500GM
Product Price: Rs 6,466 / KgGet Best Price
Product Details:
| Grade | microbiology |
| Gel Strength | Low |
| Form | Powder |
| Packaging Type | Plastic Pouch |
| Packaging Size | 500 gm |
| Brand | HIMIEDA |
| Shelf Life | 3 Years |
| Country of Origin | Made in India |
- Definition: ECD Agar is a selective medium used for detecting coliforms, especially Escherichia coli, in water, food, and other samples.
- Composition: Contains tryptone, yeast extract, bile salts, sodium chloride, phosphate salts, and agar.
- Purpose:
- Selectively isolates coliform bacteria.
- Differentiates E. coli based on indole production.
- Usage:
- Commonly used in water testing and food safety analysis.
- Utilized in membrane filter techniques.
- Preparation:
- Dissolve the medium in water, autoclave, cool, and pour into sterile Petri plates.
- Incubate samples on the medium and observe for colony growth.
- Advantages:
- Selective: Inhibits non-coliform bacteria, ensuring accurate results.
- Differential: Identifies E. coli through indole reaction (red zone around colonies).
- Reliable: Provides consistent and reproducible results.
- Applications:
- Monitoring water quality.
- Ensuring food safety.
- Conducting microbiological research.
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MacConkey Broth 500GM
Product Price: Rs 3,500 / KgGet Best Price
Product Details:
| Physical State | Powder |
| Usage/Application | Culture Media |
| Packaging Size | 500 g |
| Category | Complex Media |
| Grade Standard | Technical Grade |
| Packaging Type | Plastic Bottle |
| Country of Origin | Made in India |
| Brand | HIMIEDA |
- Purpose: MacConkey broth is a selective culture medium containing lactose.
- Function:
- Lactose degradation by bacteria produces acid and gas.
- Gas is collected in Durham tubes.
- A color change of the pH indicator bromocresol purple to yellow indicates acid production.
- Uses:
- Detection of coliform bacteria in milk and water.
- Selective enrichment of Escherichia coli from non-sterile samples
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Methylene Blue Stain Power
Product Price: Rs 800 / KgGet Best Price
Product Details:
| Form | Powder |
| Purity | 99% |
| Packaging Type | Glass Bottel |
| Packaging Size | 25G |
| Color | White |
| CAS Number | 61-73-4 |
| Brand | Rankem |
| CI Number | 52015 |
| Country of Origin | Made in India |
| Shelf Life | 24 months |
- Type: Cationic dye (positively charged)
- Color: Blue
- Usage: Staining bacteria, fungi, and other microorganisms
- Applications: Microbiology, histology, and cytology
- Function: Binds to negatively charged cell components like DNA and RNA
- Preparation: Dissolve in distilled water for use
- Storage: Keep in a dark, cool place to maintain efficacy
- Certifications: Often certified by the Biological Stain Commission
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Bismuth Sulphite Agar
Product Price: Rs 11,375 / KgGet Best Price
Minimum Order Quantity: 1 Kg
Product Brochure
Product Details:
| Grade Standard | Industrial Grade |
| Packaging Size | 1 kg |
| Usage/Application | for the isolation of Salmonella species from a variety of samples including foods and clinical speci |
| Color | light greenish-yellow |
| Country of Origin | Made in India |
- Purpose: Bismuth Sulphite Agar is a highly selective medium used for the isolation of Salmonella species, particularly S. typhi, from clinical and non-clinical specimens.
- Composition:
- Peptone: Provides carbon, nitrogen, long-chain amino acids, vitamins, and essential growth factors.
- HM Peptone B: Another source of nutrients.
- Dextrose (Glucose): Serves as the carbon source.
- Disodium phosphate: Maintains osmotic equilibrium.
- Bismuth sulfite indicator: Inhibits intestinal gram-positive and gram-negative bacteria.
- Brilliant green: Also inhibits bacterial growth.
- Agar: Solidifying agent.
- Preparation:
- Suspend 52.33 grams in 1000 ml distilled water.
- Heat to boiling to dissolve the medium completely (do not sterilize in an autoclave).
- Pour thick plates (25 ml medium per plate) and allow them to solidify.
- Correctly prepared plates should have a smooth, cream-like opacity with a pale straw color
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Reinforced Clostridial Agar
Product Price: Rs 2,800 / PackGet Best Price
Product Brochure
Product Details:
| Physical State | Semi-Solid |
| Purity | 99% |
| Category | Complex Media |
| Packaging Type | Bottle |
| Color | Yellow |
| CAS Number | 9005-25-8 |
| Boiling Point (BP) | 100 |
| Melting Point (MP) | 176 |
| Shelf Life | 4 years. |
| Country of Origin | Made in India |
-
Purpose: RC Agar is an enriched non-selective medium used for the cultivation of Clostridia and other anaerobic and facultative bacteria from various clinical and non-clinical specimens
2. Composition: It is a solid, highly nutritious medium containing ingredients that support the growth of anaerobes, lactobacilli, and other bacterial species.
3. Applications:
o Isolation and cultivation of anaerobic organisms from different habitats, including food and pathological samples.
o Enumeration of clostridia and other anaerobes.
4. Performance: RC Agar performs similarly to the pork-starch-pea agar for anaerobe counts.
5. Additional Uses:
o Investigation of intestinal flora in humans and animals.
o Anaerobic count of microorganisms from soil samples.
6. Preparation: RC Agar is available in dehydrated form and can be reconstituted with water before use
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Lactobacillus MRS Agar
Product Price: Rs 11,000 / KgGet Best Price
Product Details:
| Grade | Industry Grade |
| Form | Powder |
| Packaging Size | 500 gm |
| Packaging Type | Plastic Pouch |
| Country of Origin | Made in India |
Lactobacillus MRS Agar is a nutrient-rich, selective medium formulated by de Man, Rogosa and Sharpe (MRS) for the cultivation, enumeration, and isolation of Lactobacilli and other lactic acid bacteria from various samples including dairy, food, and probiotic products.
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Potato Dextrose Agar Powder 500GM
Product Price: Rs 1,050 / KgGet Best Price
Product Details:
| Manufacturer | Himedia |
| Form | Powder |
| Brand | Himedia |
| Shelf Life | 36 Months. |
| Country of Origin | Made in India |
| Packaging Size | 100 g |
-
- Purpose: Used for growing fungi (yeasts and molds) and some bacteria.
- Composition: Made from potato infusion, dextrose (sugar), and agar (a gelling agent).
- Preparation:
- Boil sliced potatoes in water, strain to get potato infusion.
- Mix potato infusion with dextrose and agar.
- Sterilize by autoclaving.
- Uses:
- Cultivates fungi from soil, food, and clinical samples.
- Tests for fungi in food and dairy products.
- Screens for antifungal agents in pharmaceuticals.
- Additives: Can include antibiotics or acids to inhibit bacterial growth.
- Appearance: Solid medium, usually clear to slightly yellowish.
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Potato Dextrose Agar,
Product Price: Rs 10,687 / KgGet Best Price
Product Brochure
Product Details:
| Manufacturer | HIMIEDA |
| Packaging Size | 500 g |
| Form | Powder |
| Prescription/Non prescription | Prescription |
| Brand | HIMIEDA |
| Shelf Life | 36 Months |
| Country of Origin | Made in India |
- Purpose:
- PDA is a frequently used microbial growth medium for the cultivation of molds, yeasts, and other fungi.
- It provides a rich nutritional base that encourages fungal growth.
- Composition:
- Potato Infusion: Derived from boiled potatoes, it serves as a nutrient source.
- Dextrose (Glucose): A fermentable carbohydrate.
- Agar: Added as the solidifying agent.
- Selective Properties:
- PDA can be supplemented with acid or antibiotics to inhibit bacterial growth.
- It selectively promotes the growth of fungi.
- Uses:
- Detection of yeasts and molds in dairy products and prepared foods.
- Cultivation of yeasts and molds from clinical specimens.
- Recommended for plate count methods in food testing.
- Preparation:
- Prepare potato infusion by boiling sliced, unpeeled potatoes in water.
- Mix with dextrose, agar, and water, then autoclave.
- Dispense into sterile petri dishes.
- Variants:
- PDA with TA (Tartaric Acid) inhibits bacterial growth.
- PDA with Chlortetracycline is used for microbial enumeration in cosmetics.
- PDA with Chloramphenicol selectively cultivates fungi from mixed samples
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Potato Dextrose Agar
Product Price: Rs 10,000 / KgGet Best Price
Product Details:
| Packaging Size | 500 g |
| Form | Powder |
| Braand | Himedia |
| Code | GM096 |
| Application | Cultivation of fungi and yeasts |
HiMedia's Potato Dextrose Agar (PDA), Granulated is a widely used microbiological medium for the cultivation of yeasts and molds, especially in food, dairy, and environmental microbiology. Made with dehydrated potato infusion and dextrose, it encourages robust fungal growth, making it ideal for detecting spoilage organisms and performing fungal studies.
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