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Agar Powder

Offering you a complete choice of products which include tryptone soya agar w/ added nacl, rappaport vassiliadis medium, hi crome listeria ottaviani agosti agar base, emb agar 500g, violet red bile glucose agar and mannitol salt agar.

Tryptone Soya Agar w/ added NaCl

Tryptone Soya Agar w/ added NaCl
  • Tryptone Soya Agar w/ added NaCl
  • Tryptone Soya Agar w/ added NaCl
  • Tryptone Soya Agar w/ added NaCl
  • Tryptone Soya Agar w/ added NaCl
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Approx. Price: Rs 12,550 / PackGet Latest Price
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Product Details:

Minimum Order Quantity1 Pack
Packaging Size100 g
Product CodeM1969
Brandhimedia
CAS Number7647-14-5
Shelf Life4 years
Country of OriginMade in India

  1. Purpose: A highly nutritious general-purpose medium recommended for use when 1% Sodium Chloride is needed in the medium.
  2. Composition:
    • Tryptone: 15.0 g/L
    • Soya peptone: 5.0 g/L
    • Sodium chloride: 10.0 g/L
    • Agar: 15.0 g/L
  3. Principle:
    • Tryptone and Soya peptone provide nitrogenous compounds and other growth factors.
    • The medium contains a slightly high concentration of sodium chloride, which provides essential ions and maintains osmotic equilibrium.
  4. Instructions for Use:
    • Suspend 45.0 grams in 1000 ml purified/distilled water.
    • Heat to boiling to dissolve completely.
    • Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
  5. Quality Control Specifications:
    • Appearance of powder: Yellow-colored homogeneous free-flowing powder.
    • Appearance of prepared medium: Yellow-colored clear gel forms in Petri plates.
    • pH (at 25°C): 7.3±0.2
  6. Storage: Keep the dehydrated powder in tightly-sealed containers between 25-30°C, protected from direct sunlight. Shelf life is 4 years under optimal conditions.
  7. Disposal: Sterilize prepared plates and other contaminated materials before discarding
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Rappaport vassiliadis Medium

Rappaport vassiliadis Medium
  • Rappaport vassiliadis Medium
  • Rappaport vassiliadis Medium
  • Rappaport vassiliadis Medium
  • Rappaport vassiliadis Medium
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Approx. Price: Rs 13,400 / PackGet Latest Price
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Product Details:

Physical StateSemi-Solid
Usage/ApplicationMicrobiology
Packaging Size500 g
CategorySimple Media
Grade StandardTechnical Grade
Packaging TypePlastic Bottle
Country of OriginMade in India

             Purpose: Recommended for enrichment of Salmonellae, based on its ability to multiply selectively at high osmotic pressure, low pH, and at 43°C, with modest nutritional requirements

·         Composition: Contains aluminum oxide (Al₂O₃) with impurities.

·         Applications:

o    Chromatography: Used as a buffer substance for chromatography in analytical applications.

o    Polishing: Suitable for surface finishing in ceramics, glass, and metals industries.

o    Abrasive: Effective for grinding, cutting, and shaping hard materials.

o    Catalyst Support: Used as a support material in catalysts.

·         Chemical Formula: Na₂HPO₄ · 2 H₂O

·         Molecular Weight: 177.99 g/mol

·         Density: Varies based on particle size.

·         Storage: Keep in a dry, cool place away from moisture.

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Hi Crome Listeria Ottaviani Agosti Agar Base

Hi Crome Listeria Ottaviani  Agosti Agar Base
  • Hi Crome Listeria Ottaviani  Agosti Agar Base
  • Hi Crome Listeria Ottaviani  Agosti Agar Base
  • Hi Crome Listeria Ottaviani  Agosti Agar Base
  • Hi Crome Listeria Ottaviani  Agosti Agar Base
  • Hi Crome Listeria Ottaviani  Agosti Agar Base
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Approx. Price: Rs 5,900 / PieceGet Latest Price
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Product Details:

Minimum Order Quantity1 Piece
Physical StateSemi-Solid
Purity99%
Packaging SizePlastic Bottle
Packaging TypeBottle
CategoryComplex Media
ColorWhite
Shelf Life36 MONTH
Country of OriginMade in India

    • Purpose:
    • HiCrome™ Listeria Ottaviani Agosti Agar Base is used for the selective and differential isolation of Listeria monocytogenes from food and animal feeds.
    • This medium follows the method adopted by the ISO Committee for Listeria detection 
      • Composition:
    • Contains the chromogenic compound 5-bromo-4-chloro-3-indolyl-β-D-glucopyranoside.
    • This substrate detects the presence of β-glucosidase, which is common in all Listeria species.
      •  
        • Appearance
      • Listeria colonies appear as blue-green on the medium due to β-glucosidase activity
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Emb Agar 500G

Emb Agar 500G
  • Emb Agar 500G
  • Emb Agar 500G
  • Emb Agar 500G
  • Emb Agar 500G
  • Emb Agar 500G
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Approx. Price: Rs 9,275 / PackGet Latest Price
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Product Details:

Minimum Order Quantity1 Pack
Packaging Size500 g
Manufacturerhimedia
Prescription/Non prescriptionPrescription
BrandHIMIEDA
Shelf Life36 Months.
Country of OriginMade in India

  1. Purpose:

    • EMB agar is a selective and differential culture medium used for the isolation of gram-negative bacilli.
    • It’s commonly used in microbiology labs to identify and differentiate specific bacteria.
  2. Composition:

    • Contains:
      • Peptone: Provides essential nutrients for bacterial growth.
      • Lactose: A fermentable carbohydrate.
      • Sucrose: Another fermentable sugar (though not present in all versions).
      • Eosin Y and methylene blue: pH indicator dyes.
      • Agar: Solidifying agent.
      • Dipotassium phosphate: Acts as a buffer.
  3. Selective Properties:

    • Inhibits the growth of most gram-positive organisms.
    • Promotes the growth of gram-negative bacteria.
  4. Differential Properties:

    • Differentiates bacteria based on their ability to ferment lactose:
      • Lactose fermenters: Produce acid, resulting in dark purple colonies with a green metallic sheen.
      • Slow lactose fermenters: Form brown-pink colonies.
      • Non-lactose fermenters: Produce colorless or light pink colonies.
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Violet Red Bile Glucose Agar

Violet Red Bile Glucose Agar
  • Violet Red Bile Glucose Agar
  • Violet Red Bile Glucose Agar
  • Violet Red Bile Glucose Agar
  • Violet Red Bile Glucose Agar
  • Violet Red Bile Glucose Agar
  • Violet Red Bile Glucose Agar
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Approx. Price: Rs 9,962 / PackGet Latest Price
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Product Details:

Packaging Size500 g
ManufacturerHIMEDIA
Compositionpeptone, yeast extract, sodium chloride, glucose, bile salts, crystal violet, neutral red, agar
Treatmentused for the enumeration of Enterobacteriaceae in foods
Prescription/Non prescriptionPrescription
Final pH7.4
Product CodeMH581
BrandHIMEDIA
CAS Number302-95-4
Shelf Life36 Months
Country of OriginMade in India

1.    Purpose: VRBGA is a selective medium used for the detection and enumeration of bile-tolerant Gram-negative bacteria in various samples, including food, water, and other materials of sanitary importance 

2.    Composition:

o    Peptic digest of animal tissue: Provides carbon, nitrogen, vitamins, and other essential growth nutrients.

o    Yeast extract: Additional source of nutrients.

o    Sodium chloride: Maintains osmotic equilibrium.

o    Bile salts mixture: Inhibits accompanying Gram-positive flora.

o    Crystal violet: Further inhibits Gram-positive organisms, especially Staphylococci.

o    Glucose: The fermentable carbohydrate.

o    Neutral red: Acts as a pH indicator 

3.    Result Interpretation:

o    Purple colonies surrounded by purple haloes: Rapid lactose fermenters (e.g., coliform bacteria like E. coli).

o    Pale colonies with greenish zones: Non-fermenters or late lactose fermenters.

o    Pink pin-point colonies: Possibly Enterococci

o    Colorless colonies: Lactose-negative Bacteria 

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Mannitol Salt Agar

Mannitol Salt Agar
  • Mannitol Salt Agar
  • Mannitol Salt Agar
  • Mannitol Salt Agar
  • Mannitol Salt Agar
  • Mannitol Salt Agar
  • Mannitol Salt Agar
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Approx. Price: Rs 2,160 / PackGet Latest Price
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Product Details:

Packaging Size100 g
ManufacturerHIMIEDA
Treatmentisolating pathogenic staphylococci from samples,cosmetics,and microbial limit tests.
Prescription/Non prescriptionPrescription
Final pH7.4
Product CodeMH118
BrandHIMIEDA
CAS Number143-74-8
Shelf Life36 MONTHS
Country of OriginMade in India

 

·         Purpose: MSA is a commonly used selective and differential growth medium in microbiology.

·         Selective Action:

·         Contains 7.5% sodium chloride, which selects for bacteria that can tolerate high salt concentrations.

·         Staphylococci species can grow on MSA due to their salt tolerance.

·         Inhibits the growth of most other gram-positive and gram-negative bacteria.

·         Differential Aspect:

·         Staphylococcus aureus (pathogenic staphylococci) can ferment mannitol, changing the pH of the medium to acidic.

·         MSA contains phenol red as a pH indicator.

·         Acidic pH turns the medium yellow due to mannitol fermentation by S. aureus.

·         Coagulase-negative staphylococci (CONS) do not ferment mannitol, so the medium remains pink around their colonies.

·         Composition:

·         Enzymatic digest of casein, animal tissue, and beef extract (sources of nutrients).

·         D-Mannitol as the sole carbohydrate source.

·         Sodium chloride (selective agent).

·         Phenol red (pH indicator).

·         Agar (solidifying agent).

·         Application: Used to isolate and differentiate Staphylococcus spp., particularly S. aureus

 

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Orange Serum Agar

Orange Serum Agar
  • Orange Serum Agar
  • Orange Serum Agar
  • Orange Serum Agar
  • Orange Serum Agar
  • Orange Serum Agar
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Approx. Price: Rs 12,187 / PackGet Latest Price
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Product Details:

Packaging Size500 g
ManufacturerHIMEDIA
Prescription/Non prescriptionPrescription
Final pH5.4 - 5.6
Product CodeM933
BrandHIMIEDA
CAS Number110673.
Shelf Life36 Months.
Country of OriginMade in India

    • Purpose: Orange Serum Agar is specifically designed for the isolation and enumeration of microorganisms that can survive in citrus products.
    • Microorganisms Targeted: It helps detect acid-tolerant bacteria, molds, and yeasts commonly associated with spoilage in citrus fruit products.
    • Low pH Environment: The low pH of citrus juices limits the growth of microorganisms to those capable of tolerating the acidic conditions.
    • Composition:
      • Tryptone: Provides amino acids and complex nitrogenous substances.
      • Glucose: Serves as a fermentable carbohydrate and energy source.
      • Yeast extract: Supplies vitamins to stimulate growth.
      • Orange serum: Creates an optimal environment for acid-tolerant microorganisms.
      • Superiority: In comparative studies, Orange Serum Agar was found to be superior to other media for total count and detection of spoilage organisms1.
      • Application: Used in laboratories to cultivate and enumerate acidic microorganisms associated with citrus product spoilage
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Tergitol-7 Agar Base

Tergitol-7 Agar Base
  • Tergitol-7 Agar Base
  • Tergitol-7 Agar Base
  • Tergitol-7 Agar Base
  • Tergitol-7 Agar Base
  • Tergitol-7 Agar Base
  • Tergitol-7 Agar Base
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Approx. Price: Rs 12,925 / PackGet Latest Price
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Product Details:

Packaging Size500 g
ManufacturerHIMIEDA
Prescription/Non prescriptionPrescription
Final pH6.50 - 7.50
Storage Temperaturebelow 30 DegreeC
Product CodeM616
BrandHIMIEDA
Melting Temp38-40C
CAS Number9002-18-0
Shelf Life36 Months.
Country of OriginMade in India

1.    Purpose: Tergitol-7 Agar Base is used for selective enumeration and identification of coliform organisms.

2.    Selective Medium: It helps detect coliform bacteria and allows for early detection of E. coli in water analysis.

3.    Composition: The base contains Sodium heptadecyl sulphate (Tergitol-7), which inhibits gram-positive bacteria.

4.    Modification: Chapman modified the original formula by adding Triphenyl Tetrazolium Chloride (TTC), making the medium selective and differential1.

5.    Recommended Use: Tergitol-7 Agar Base is recommended by the ISO Committee for coliform detection and enumeration

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Columbia Agar 500G

Columbia Agar 500G
  • Columbia Agar 500G
  • Columbia Agar 500G
  • Columbia Agar 500G
  • Columbia Agar 500G
  • Columbia Agar 500G
  • Columbia Agar 500G
  • Columbia Agar 500G
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Approx. Price: Rs 11,637 / KgGet Latest Price
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Product Details:

Minimum Order Quantity1 Kg
Packaging Size500 g
ManufacturerHIMIEDA
Treatmentused with or without blood for the isolation and cultivation of a wide variety of fastidious microor
Final pH7.3
Storage Temp10-30 DegreeC
Product CodeDM115D
BrandHIMIEDA
Melting Temp45 - 50 DegreeC.
CAS Number9005-84-9.
Shelf Life1095 days
Country of OriginMade in India

  • Purpose: Bismuth Sulphite Agar is a highly selective medium used for the isolation of Salmonella species, particularly S. typhi, from clinical and non-clinical specimens.
  • Composition:
    • Peptone: Provides carbon, nitrogen, long-chain amino acids, vitamins, and essential growth factors.
    • HM Peptone B: Another source of nutrients.
    • Dextrose (Glucose): Serves as the carbon source.
    • Disodium phosphate: Maintains osmotic equilibrium.
    • Bismuth sulfite indicator: Inhibits intestinal gram-positive and gram-negative bacteria.
    • Brilliant green: Also inhibits bacterial growth.
    • Agar: Solidifying agent.
  • Detection Mechanism:
    • S. TyphiS. Enteritidis, and S. Typhimurium typically grow as black colonies with a surrounding metallic sheen due to hydrogen sulfide production and reduction of sulfite to black ferric sulfide.
    • Salmonella Paratyphi A grows as light green colonies.
  • Preparation:
    • Suspend 52.33 grams in 1000 ml distilled water.
    • Heat to boiling to dissolve the medium completely (do not sterilize in an autoclave).
    • Pour thick plates (25 ml medium per plate) and allow them to solidify.
    • Correctly prepared plates should have a smooth, cream-like opacity with a pale straw color
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Simmons Citrate Agar

Simmons Citrate Agar
  • Simmons Citrate Agar
  • Simmons Citrate Agar
  • Simmons Citrate Agar
  • Simmons Citrate Agar
  • Simmons Citrate Agar
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Approx. Price: Rs 10,175 / KgGet Latest Price
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Product Details:

Packaging DetailsPlastic Bottle
Purity99%
Packaging Size500 gm
Packaging TypeBottle
Usage/ApplicationLaboratory
BrandHIMIEDA
CAS Number7722-76-1.
Shelf Life60 Month
Country of OriginMade in India

  • Purpose:
    • Simmons Citrate Agar is a differential medium that helps distinguish bacteria based on their ability to utilize citrate as their sole source of carbon.
    • It is commonly used in laboratories to gain insights into the metabolic capabilities of different bacterial species.
  • Composition:
    • Contains sodium citrate as the sole carbon source.
    • Ammonium dihydrogen phosphate serves as the sole nitrogen source.
    • Other components include sodium chloride, dipotassium phosphate, magnesium sulfate, bromothy mol blue (pH indicator), and agar (solidifying agent).
  • Principle:
    • Organisms capable of utilizing citrate will grow unrestricted on this medium.
    • Bacteria that can metabolize citrate produce an enzyme called citrate-permease, which converts citrate to pyruvate.
    • Pyruvate then enters the organism’s metabolic cycle for energy production.
    • Growth on Simmons Citrate Agar indicates utilization of citrate.
  • Uses:
    • Differentiating Gram-negative bacteria based on citrate utilization.
    • Identifying citrate-positive Salmonella species from citrate-negative ones.
    • Aiding in the identification of Entero bacteriaceae.
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Chromogenic Coliform Agar

Chromogenic Coliform Agar
  • Chromogenic Coliform Agar
  • Chromogenic Coliform Agar
  • Chromogenic Coliform Agar
  • Chromogenic Coliform Agar
  • Chromogenic Coliform Agar
  • Chromogenic Coliform Agar
  • Chromogenic Coliform Agar
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Approx. Price: Rs 84,187 / KgGet Latest Price
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Product Details:

Minimum Order Quantity1 Kg
Packaging Size500 g
ManufacturerHimedia
Compositionenzymatic digest of casein, yeast extract, sorbitol and sodium pyruvate
Treatmentdrinking water, disinfected pool water or finished water from treatment plants.
Prescription/Non prescriptionPrescription
Final pH6.8
Storage Temperature15-25 DegreeC
Storage Temp2-8 DegreeC
Product CodeM1991i
BrandHIMIEDA
CAS Number1473387-52-8
Shelf Life24 Months
Country of OriginMade in India

  • Purpose: CCA is a selective agar medium used to detect coliform bacteria in water and other samples.
  • Differentiation: It contains chromogenic substrates that allow for distinguishing between coliform and non-coliform bacteria based on their ability to produce specific enzymes.
  • Enzyme Indicators:
    • Coliform Bacteria: Produce β-D-galactosidase, resulting in pink to red colonies.
    • E. coli: Produce β-D-glucuronidase, leading to dark blue to violet colonies.
  • Composition (per liter):
    • Tryptone: 1 g
    • Yeast extract: 2 g
    • Sodium chloride: 5 g
    • Sodium dihydrogen phosphate: 2.2 g
    • Disodium hydrogen phosphate: 2.7 g
    • Sodium pyruvate: 1 g
    • Sorbitol: 1 g
    • 6-chloro-3-indoxyl β-D-galactopyranoside: 0.2 g
    • 5-bromo-4-chloro-3-indoxyl β-D-glucuronic acid: 0.1 g
    • IPTG (Isopropyl-β-D-thiogalactopyranoside): 0.1 g
    • Agar: 15 g
    • Final pH: 6.8±0.2 at 25°C1.
  • Application: Used for microbiological quality testing of water and food samples to detect coliforms
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Special Infusion Agar

Special Infusion Agar
  • Special Infusion Agar
  • Special Infusion Agar
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Approx. Price: Rs 2,162.5 / KgGet Latest Price
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Product Details:

Minimum Order Quantity1 Kg
Packaging Size100 g
Product CodeM211
Melting Temp90C
BrandHIMEDIA
CAS Number7558-79-4
Shelf Life4 years
Country of OriginMade in India

  • Purpose:
    • BHI Agar is a general-purpose medium used for the primary isolation of aerobic bacteria from clinical specimens.
    • It is highly nutritious and can support luxuriant growth of a wide variety of microorganisms.
  • Composition:
    • BHI Agar derives its nutrients from brain heart infusionpeptone, and glucose components.
    • Protease peptone and infusions serve as sources of carbonnitrogenvitaminsamino acids, and essential growth factors.
  • Enrichment and Selectivity:
    • BHI Agar can be further enriched by the addition of blood.
    • It can also be rendered selective by adding different antibiotics.
  • Applications:
    • Cultivation of fastidious pathogenic bacteriayeasts, and moulds.
    • Widely used in clinical laboratories for bacterial isolation
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Shigella broth Base

Shigella broth Base
  • Shigella broth Base
  • Shigella broth Base
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Approx. Price: Rs 11,610 / PackGet Latest Price

Product Details:

Minimum Order Quantity1 Pack
Physical StatePowder
Usage/ApplicationMicrobiology
Packaging Size500 g
Categoryenrichment media.
Grade StandardAnalytical Grade
Packaging TypePlastic Bottle
Country of OriginMade in India

  1. Chemical Name: Shigella Broth Base
  2. Use: Isolation and cultivation of Shigella species from food
  3. Composition:
    • Contains casein enzymic hydrolysate as a source of carbon, nitrogen, vitamins, and minerals
    • Dextrose provides necessary carbohydrates
    • Buffering by dipotassium hydrogen phosphate and potassium dihydrogen phosphate
    • Sodium chloride maintains osmotic balance of the medium
  4. Safety: Handle with care; avoid contact with skin and eyes
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Baird Parker Agar Base

Baird Parker Agar Base
  • Baird Parker Agar Base
  • Baird Parker Agar Base
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Approx. Price: Rs 12,325 / PackGet Latest Price

Product Details:

Minimum Order Quantity1 Pack
Packaging Size500 g
ManufacturerHImedia
CompositionTryptone, HM peptone B and yeast extract are sources of nitrogen, carbon, sulphur, and vitamins.
Treatmentnot intended for use in diagnosis, treatment, or prevention of disease in humans
Product CodeCM0961B
Melting Temp100C
BrandHImedia
CAS Number147-14-8
Shelf Life4 years
Country of OriginMade in India

·         Chemical Name: Baird Parker Agar Base

·         Use: Selective enumeration medium for Staphylococcus aureus.

·         Composition:

·         Tryptone: Provides nitrogen, carbon, sulfur, and vitamins.

·         HM Peptone B: Source of nutrients.

·         Yeast extract: Supplies carbon, nitrogen, and vitamins.

·         Glycine: Enhances Staphylococcus growth.

·         Sodium pyruvate: Stimulates S. aureus growth.

·         Lithium chloride: Inhibits most contaminants.

·         Agar: Solidifying agent.

·         Principle: Correlates coagulase test with lipolysis zone; black colonies indicate tellurite reduction by coagulase-positive Staphylococci.

·         Result Interpretation: Grey-black colonies and a clear zone are diagnostic for coagulase-positive Staphylococci.

·         Preparation: Dissolve 63.0 grams in 950 ml distilled water, sterilize, and add egg yolk emulsion and potassium tellurite solution

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Bhi Broth 500GM

Bhi Broth 500GM
  • Bhi Broth 500GM
  • Bhi Broth 500GM
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Approx. Price: Rs 9,450 / PackGet Latest Price

Product Details:

Minimum Order Quantity1 Pack
Physical StatePowder
Usage/ApplicationMicrobiology
Packaging Size500 g
CategorySimple Media
Grade StandardTechnical Grade
Packaging TypePlastic Bottle
Country of OriginMade in India

·         Chemical Name: BHI Broth (Brain Heart Infusion Broth)

·         Use: Highly nutritive medium for cultivating a wide variety of microorganisms.

·         Applications:

o   Preparation of inocula for antimicrobial susceptibility testing.

o   Preferred medium for anaerobic bacteriayeasts, and other microorganisms.

·         Composition:

o   Contains casein enzymic hydrolysatedextrose, and buffering agents.

·         Safety: Handle with care; avoid contact with skin and eyes.

·         Storage: Store appropriately to maintain stability

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Buffered Sodium Chloride Peptone Solution

Buffered Sodium Chloride Peptone Solution
  • Buffered Sodium Chloride Peptone Solution
  • Buffered Sodium Chloride Peptone Solution
  • Buffered Sodium Chloride Peptone Solution
  • Buffered Sodium Chloride Peptone Solution
  • Buffered Sodium Chloride Peptone Solution
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Approx. Price: Rs 2,090 / KgGet Latest Price
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Product Details:

Minimum Order Quantity1 Kg
Purity99%
GradeIndustrial
Usage/Applicationas a diluent for carrying out microbial limit test from pharmaceutical products
Molecular Weight58.44
Packaging TypePlastic Bottle
Country of OriginMade in India
FormPowder

  • Purpose: Buffered Sodium Chloride Peptone Solution is primarily used for microbial limit testing in pharmaceutical products.
  • Composition: The solution contains sodium chloride, peptone, and a phosphate buffer. The low peptone content helps maintain the viability of microorganisms without supporting their growth 
  • Applications:
    • Microbial Examination: Used for the microbial examination of non-sterile pharmaceutical products 
    • Sample Preparation: Can be used as a solution for sample preparation, dilution, or washing
    • Cosmetics: Conforms to ISO 21149 for the detection of viable microorganisms in cosmetics

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Lactobacillus MRS Agar (MRS Agar)

Lactobacillus MRS Agar (MRS Agar)
  • Lactobacillus MRS Agar (MRS Agar)
  • Lactobacillus MRS Agar (MRS Agar)
  • Lactobacillus MRS Agar (MRS Agar)
  • Lactobacillus MRS Agar (MRS Agar)
  • Lactobacillus MRS Agar (MRS Agar)
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Approx. Price: Rs 11,350 / GramGet Latest Price
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Product Details:

Packaging Size500 gm
GradeFood Grade
FormPowder
Shelf Life3 Years
ColorBrown
Usage/Applicationisolation and enumeration of lactic acid bacteria from meat and meat products
BrandHIMIEDA
Country of OriginMade in India

1.    Purpose: Lactobacillus MRS Agar is a medium used for the selective isolation and enumeration of Lactobacilli from various sources, including feces and dairy.

2.    Composition:

o    Proteose Peptone, Beef Extract, and Yeast Extract provide nitrogenous sources, carbohydrates, vitamins, and minerals.

o    Glucose serves as the primary carbohydrate.

o    Other components include dipotassium phosphate, sodium acetate, triammonium citrate, magnesium sulfate, manganese sulfate, Tween® 80, and agar.

3.    Development: Originally developed in 1960 by de Man, Rogosa & Sharpe, this medium is suitable for most lactic acid bacteria and serves as a substitute for Tomato Juice Agar.

4.    pH Adjustment: When acidified to pH 5.4, it can be used to enumerate Lactobacillus bulgaricus in yogurts.

5.    Selectivity: Ammonium citrate and sodium acetate provide selectivity against streptococci and molds.

6.    Incubation: Incubate agar plates at 35°C for 3 days or at 30°C for 5 days in an aerobic atmosphere supplemented with carbon dioxide

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Potato Dextrose Agar,

Potato Dextrose Agar,
  • Potato Dextrose Agar,
  • Potato Dextrose Agar,
  • Potato Dextrose Agar,
  • Potato Dextrose Agar,
  • Potato Dextrose Agar,
  • Potato Dextrose Agar,
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Approx. Price: Rs 10,687 / KgGet Latest Price
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Product Details:

ManufacturerHIMIEDA
Packaging Size500 g
FormPowder
Prescription/Non prescriptionPrescription
BrandHIMIEDA
Shelf Life36 Months
Country of OriginMade in India

  • Purpose:
    • PDA is a frequently used microbial growth medium for the cultivation of molds, yeasts, and other fungi.
    • It provides a rich nutritional base that encourages fungal growth.
  • Composition:
    • Potato Infusion: Derived from boiled potatoes, it serves as a nutrient source.
    • Dextrose (Glucose): A fermentable carbohydrate.
    • Agar: Added as the solidifying agent.
  • Selective Properties:
    • PDA can be supplemented with acid or antibiotics to inhibit bacterial growth.
    • It selectively promotes the growth of fungi.
  • Uses:
    • Detection of yeasts and molds in dairy products and prepared foods.
    • Cultivation of yeasts and molds from clinical specimens.
    • Recommended for plate count methods in food testing.
  • Preparation:
    • Prepare potato infusion by boiling sliced, unpeeled potatoes in water.
    • Mix with dextrose, agar, and water, then autoclave.
    • Dispense into sterile petri dishes.
  • Variants:
    • PDA with TA (Tartaric Acid) inhibits bacterial growth.
    • PDA with Chlortetracycline is used for microbial enumeration in cosmetics.
    • PDA with Chloramphenicol selectively cultivates fungi from mixed samples
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Reinforced Clostridial Agar

Reinforced Clostridial Agar
  • Reinforced Clostridial Agar
  • Reinforced Clostridial Agar
  • Reinforced Clostridial Agar
  • Reinforced Clostridial Agar
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Approx. Price: Rs 2,800 / PackGet Latest Price
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Product Details:

Physical StateSemi-Solid
Purity99%
CategoryComplex Media
Packaging TypeBottle
ColorYellow
CAS Number9005-25-8
Boiling Point (BP)100
Melting Point (MP)176
Shelf Life4 years.
Country of OriginMade in India

  1.       Purpose: RC Agar is an enriched non-selective medium used for the cultivation of Clostridia and other anaerobic and facultative bacteria from various clinical and non-clinical specimens

    2.      Composition: It is a solid, highly nutritious medium containing ingredients that support the growth of anaerobes, lactobacilli, and other bacterial species.

    3.      Applications:

    o    Isolation and cultivation of anaerobic organisms from different habitats, including food and pathological samples.

    o    Enumeration of clostridia and other anaerobes.

    4.      Performance: RC Agar performs similarly to the pork-starch-pea agar for anaerobe counts.

    5.      Additional Uses:

    o    Investigation of intestinal flora in humans and animals.

    o    Anaerobic count of microorganisms from soil samples.

    6.      Preparation: RC Agar is available in dehydrated form and can be reconstituted with water before use

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Bismuth Sulphite Agar

Bismuth Sulphite Agar
  • Bismuth Sulphite Agar
  • Bismuth Sulphite Agar
  • Bismuth Sulphite Agar
  • Bismuth Sulphite Agar
  • Bismuth Sulphite Agar
  • Bismuth Sulphite Agar
  • Bismuth Sulphite Agar
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Approx. Price: Rs 11,375 / KgGet Latest Price
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Product Details:

Minimum Order Quantity1 Kg
Grade StandardIndustrial Grade
Packaging Size1 kg
Usage/Applicationfor the isolation of Salmonella species from a variety of samples including foods and clinical speci
Colorlight greenish-yellow
Country of OriginMade in India

  • Purpose: Bismuth Sulphite Agar is a highly selective medium used for the isolation of Salmonella species, particularly S. typhi, from clinical and non-clinical specimens.
  • Composition:
    • Peptone: Provides carbon, nitrogen, long-chain amino acids, vitamins, and essential growth factors.
    • HM Peptone B: Another source of nutrients.
    • Dextrose (Glucose): Serves as the carbon source.
    • Disodium phosphate: Maintains osmotic equilibrium.
    • Bismuth sulfite indicator: Inhibits intestinal gram-positive and gram-negative bacteria.
    • Brilliant green: Also inhibits bacterial growth.
    • Agar: Solidifying agent.
  • Preparation:
    • Suspend 52.33 grams in 1000 ml distilled water.
    • Heat to boiling to dissolve the medium completely (do not sterilize in an autoclave).
    • Pour thick plates (25 ml medium per plate) and allow them to solidify.
    • Correctly prepared plates should have a smooth, cream-like opacity with a pale straw color
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